| Serie 1 |
| Mayerli Ibeth Minda Meneses | FD Carchi | 4 | 1:01.90 | 1 q |
| Valentina Paredes Torres | FD Guayas | 3 | 1:02.45 | 2 q |
| Maria Belen Morejon Quiñonez | FD Imbabura | 7 | 1:12.07 | 7 q |
| Luisana Marin Vargas | Lycans Athletics | 6 | 1:22.96 | 9 |
| Doménica Nicole Flores Vélez | FD Santa Elena | 5 | DNS | |
| Serie 2 V.V. +0.3 |
| Genesis Nicole Cañola Pluas | FD Guayas | 3 | 1:02.54 | 3 q |
| Camille Paulete Romero Carvajal | CD Pichincha | 7 | 1:02.58 | 4 q |
| Dayanari Anai Benavides Abregon | FD Guayas | 4 | 1:03.68 | 5 q |
| Scarlet Rosado Briones | Lycans Athletics | 6 | 1:07.25 | 6 q |
| Samantha Abigail Blacio Espinoza | Lycans Athletics | 5 | 1:16.04 | 8 q |
