| Serie 1 |
| Anahi Gabriela Suárez Suárez | CD Pichincha | 4 | 52.56 | 1 q |
| Rose Mary Almanza Blanco | Cuba | 7 | 52.66 | 2 q |
| Anabel Medina Ventura | República Dominicana | 8 | 53.50 | 3 q |
| Nicole Dayci Caicedo Arce | FD Guayas | 5 | 54.11 | 4 q |
| Cisne Katherine Ureña Rodriguez | FD Loja | 3 | 1:02.44 | 9 |
| Mikeiry Noemi Moreno Rodrigurz | República Dominicana | 6 | DNS | |
| Serie 2 V.V. -0.5 |
| Andreina Samantha Minda Valencia | CD Pichincha | 8 | 54.75 | 5 q |
| Virginia Villalva Perea | CD Pichincha | 5 | 55.74 | 6 q |
| Zulley Melissa Torres Vásquez | Colombia | 7 | 55.80 | 7 q |
| Evelin Jimena Mercado Hurtado | CD Pichincha | 4 | 57.95 | 8 q |
| Katherine Piedad Morejon Quiñonez | FD Imbabura | 3 | DNS | |
| Anais Maribel Lara Borja | FD Carchi | 6 | DNS | |
