| Serie 1 V.V. +0.7 |
| Genesis Nicole Cañola Pluas | FD Guayas | 4 | 26.74 | 2 q |
| Tanya Milena Hugo Mancilla | Rolando Saquipay | 3 | 27.42 | 5 q |
| Camille Paulete Romero Carvajal | CD Pichincha | 5 | 27.73 | 6 q |
| Rihanna Morales Minda | CD Pichincha | 7 | 29.43 | 8 q |
| Dayranna Valentina Muñoz Valarezo | Lycans Athletics | 6 | 30.99 | 9 |
| Serie 2 V.V. -0.8 |
| Marolyn Ariana Pastrana Flores | CD Pichincha | 4 | 26.01 | 1 q |
| Dayanna Mirelly Sanchez Mite | Lycans Athletics | 5 | 27.17 | 3 q |
| Ana De Jesús Wilson Pujupat | FD Guayas | 3 | 27.27 | 4 |
| Scarlet Nayely Giron Cuenca | FD Loja | 7 | 28.97 | 7 q |
| Josselyn Rebeca Jimenez Rosales | FD Loja | 6 | DNS | |
